Electron Microscopy Senior\Junior EM Specialists job with Human Technopole

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APPLICATION CLOSING DATE: September 1st 2023

About the Institute

Human Technopole (HT) is a new interdisciplinary life science research institute, created and supported by the Italian government, with the aim of developing innovative strategies to improve human health. HT has the mission of conducting cutting-edge research and translate innovation into methods available to the entire National scientific community. To this end, HT is opening National Facilities that will provide state-of-the-art research infrastructure for Structural Biology, Light Imaging, Genomics, Genome Engineering, and Data Handling and Analysis. In this context, the Cryo-Electron Microscopy (Cryo-EM) Unit, as part of the National Facility for Structural Biology, seeks to recruit two Senior and\or Junior Electron Microscopy (EM) Specialists that will actively participate in maintaining the national facility, take part in scientific collaborations, and support internal and external users.

About the Cryo-EM Unit

The Cryo-EM Unit workflows span from sample preparation (i.e. negative staining, plunge freezing, high-pressure freezing, freeze substitution, chemical fixation, resin embedding, carbon coating and metal sputtering, cryo-ultramicrotomy, and micropatterning) to imaging techniques (i.e. TEM\STEM imaging at room and cryogenic temperatures on side-entry holder systems, widefield and confocal cryo-CLEM imaging, SEM imaging and FIB-milling, TEM\EF-TEM imaging at cryogenic temperatures on autoloader-equipped systems). Currently, the Cryo-EM Unit owns: a 300kV Thermo Scientific Titan Krios G4i TEM with Falcon 4i, Selectris X energy filter, and Volta phase-plate; a 200kV Thermo Scientific Glacios TEM with Falcon 4i, Selectris X energy filter, Volta phase-plate, and micro-ED package; a 300kV Thermo Scientific Spectra S/TEM with CETA 2 with speed enhancement, HAADF, BF and DF detectors; a 120kV Thermo Scientific Talos L120C TEM with Gatan Elsa cryo-holder; a Thermo Scientific Aquilos 2 DualBeam cryo-FIB system with EasyLift system, AutoSlice-and-View and AutoTEM modules; a Leica Thunder widefield and a Leica Stellaris confocal microscopes with white light laser equipped with cryo-stage for CLEM applications; all ancillary sample preparation equipment including plunge freezing devices, glow discharger units, plasma cleaners, carbon and metal sputter coater, high-pressure freezer, automated freeze substitution device, micropatterning device and ultramicrotome for both resin-embedded and vitrified samples. Our microscopes operate with SerialEM, Thermo Scientific EPU, Tomography, EPU-D, Velox and MAPS. Computing resources for EM-related analyses software packages are also available via VMs and HPC. Expansion of the Cryo-EM Unit with more high-end microscopes and equipment is planned from 2024.

Key tasks and responsibilities

While building a solid expertise in all the above-mentioned techniques, the activity of each Senior\Junior EM Specialist will particularly focus on one of the following applications:

Senior\Junior EM Specialist will report directly to Head of the National Facility for Structural Biology and will work in close collaboration with other Units of the National Facility and research groups of the Structural Biology Research Centre.

The Senior\Junior EM Specialist will:

Job requirements

Mandatory Requirements

Preferential requirements

Organizational and social skills:

Application instructions

Please apply by sending:

Any questions concerning the role and/or queries regarding the relevant terms and conditions should be addressed to [email protected] (this email address should not be used to send applications).Why Human Technopole

Main benefits

Special consideration will be given to candidates who are part of the protected categories list, according to L. 68/99.

Number of positions offered: 2

Contract offered: CCNL Chimico Farmaceutico, Fixed-term 3 years - employee level.

The position is based in Milan.

Human Technopole is a new international research institute focused on life sciences, located at the heart of MIND Milano Innovation District. Our overarching mission is to improve human health and well-being by carrying out research in life sciences to develop novel approaches in preventive and personalised medicine. HT will train the next generation of leading scientists, and by setting up scientific services and facilities equipped with cutting-edge technology and scientific expertise, the institute will be at service to the national and international scientific community, promoting innovation and progress through technology transfer. Once fully operational, HT will employ over 1.000 scientists from all over the world.

At Human Technopole we offer the opportunity to be part of a new, dynamic research institute promoting a highly collaborative and international working environment. We aim to attract the best resources in each field with a selection based on merit and transparency as we believe that highly diverse teams produce the best and most innovative results.

If you are a passionate person who likes to seize great challenges, consider applying for one of our scientific or administrative vacancies.

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